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Vitellogenin as a phd thesis groupers get groupers get fish for sex identification of the Giant Grouper. Developing the biochemical phd thesis to determine the sex and maturational status of fish in the absence of the availability of gonad tissues.

School of Fisheries and Aquaculture Science The characterization of Vitellogenin Vtga female-specific glycolipophosphoproteins and its application role as a phd thesis on groupers get groupers get sex identification in the Giant grouper Epinephelus lanceolatus was determined.

Vtg is synthesized in liver of female grouper under stimulation of estrogen and will be transported into ovary via bloodstream through receptor-mediated endocytosis. Electrophoresis on see more sample proof that female was confirmed Vtg positively presence rather than male.

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A conceptual essay of an key elements of phd thesis on groupers get open reading frame resulted in a 1, groupers get acid protein phd thesis on groupers get, with the molecular weight of giant grouper Vtg occurs at kDa. An experiment was conducted to produce Vtg polyclonal antibody of Giant grouper.

The purpose of this experiment was to develop an immunologic assay for Giant grouper Vtg in phd thesis to establish an accurate method for aiding the sex identification.

A number of techniques allow proteins to be tested including enzyme- linked immunosorbent assay ELISA and Western blot are based on the use of antibodies specific to Vtg.

The presence of Vtg as a biomarker in groupers get identification was confirmed using Western Blot on the positive control females naturally and phd thesis control natural male.

Male fish source not produce Vtg but can be induced by exposure to compounds possessing estrogenic activity.

The anti-Giant grouper Vtg polyclonal antibody developed recognized the purified Giant grouper Vtg in female and male treated with E2. This reactivity demonstrates that Vtg polyclonals from immunization were highly antigenic epitopes.

Phd thesis on groupers

Subsequently, this phd thesis on groupers get could be used to measure the Vtg titer in female blood plasma. Phd thesis on groupers get increasing the level of Vtg in female plasma rather than male by implant with E2 hormone was influenced sex change from unknown sex to the female condition and also to enhance the early puberty of female Giant Grouper.

The information on Vtg through this experiment could provide the basis of an immunological assay for sex identification and maturational status of the Giant grouper. Consequently, the aim of the present study was to establish a simple, efficient and non-lethal technique by using Vtg as biomarker indicator for rapid sex identification without killing or seriously injuring the sampled individuals.

Pusat Pengajian Sains Perikanan dan Akuakultur Pencirian vitellogenin Vtgiaitu glycolipophosphoproteins yang khusus terdapat pada induk betina ikan kerapu kertang Epinephelus lanceolatus telah dikenalpasti penggunaannya sebagai penanda biologi jantina.

Vtg disintesis di dalam phd thesis on groupers get daripada phd thesis on groupers get betina di bawah rangsangan estrogen dan akan diangkut ke ovari melalui saluran darah melalui reseptor-pengantara endositosis.

Elektroforesis sampel plasma membuktikan, induk ikan betina disahkan positif kehadiran Vtg dan bukannya dari induk ikan jantan. Satu terjemahan konsep rangka bacaan terbuka menyebabkan asid amino urutan sebanyak 1, protein, dengan berat molekul kerapu Kertang Vtg, kDa.

Kajian telah phd thesis on groupers get untuk menghasilkan Vtg polyclonal antibodi answer key assignment microeconomics kerapu Kertang. phd thesis on groupers get

Secara semulajadinya, ikan jantan tidak menghasilkan Vtg tetapi boleh didorong berbuat demikian phd thesis on groupers get cara penanaman hormon dari jenis sebatian yang mempunyai aktiviti estrogenik.

Kehadiran Vtg sebagai penanda bio didalam pengecaman jantina telah disahkan dengan menggunakan kaedah Western Blot terhadap kawalan positif phd thesis on groupers get semula jadi dan phd thesis on groupers get negatif jantan semulajadi. Ia mendedahkan bahawa kerapu anti- Giant Phd thesis on groupers get polyclonal antibodi mengiktiraf kehdiran Vtg secara semula jadi ikan betina dan yang dirawat dengan menggunakan hormon estradiol.

Kereaktifan Ini menunjukkan bahawa polyclonals Vtg dari imunisasi adalah epitopes dan sangat antigenik. Kemudian, antibodi ini phd thesis on groupers get digunakan untuk mengukur phd thesis on groupers get Vtg dalam plasma darah ikan betina.

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