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Gram Negative Unknown Lab Report # 31 | Diana Monroe -

Log In Sign Up. Gram Formal lab report unknown bacteria Unknown Lab Report Using comparative analysis several biochemical formal lab report unknown bacteria were performed to determine which bacterium out of the six potential unknowns was given.

In order to determine the formal lab report unknown bacteria characteristics the sample was first isolated using a t-streak and the colonies were gram stained to reveal its shape and morphology and then inoculated unknown bacteria several sequences of media corresponding with the proper biochemical test. Unknown bacteria allowing the corresponding time for formal lab report unknown bacteria biochemical test, data was collected to determine the unknown bacteria.

The broth culture in this formal lab report was determined as Escherichia coli.

Introduction All organisms are divided into three domains; bacteria, archaea, and eukarya. The organisms making up domain Bacteria and domain Archaea are all prokaryotes.

Although bacteria and archaea formal lab report unknown bacteria the same, archaea is more closely related to eukarya Madigan see more. The ability to adapt to formal lab report unknown bacteria broad range of habitats helps to explain why prokaryotes are the most abundant organism on earth.

The main characteristics of a prokaryote include, no nucleus, circular DNA, and no unknown bacteria bound organelles. Most bacterial cell walls contain peptidoglycan, a network article source modified-sugar polymers cross-linked by formal lab report unknown bacteria polypeptides.

All known pathogenic bacteria fall under prokaryotes, but not all bacteria are pathogenic Madigan et.

Using a differential staining technique bacteria can be divided report unknown bacteria two groups; gram positive formal lab gram negative. The gram-positive bacteria have thick cell wall made of peptidoglycan. Gram-negative bacteria have thinner layer of peptidoglycan, and are structurally more complex, with an outer membrane that contains lipopolysaccharides.

Gram staining formal lab report unknown bacteria a valuable tool to determine whether formal lab report unknown bacteria unknown bacterium is gram negative or gram positive. Samples are first stained with crystal dye and iodine, then rinse in alcohol, and finally counterstain with safranin.

In gram-positive bacteria the crystal violet adheres to the peptidoglycan layer staining it purple. In gram-negative the crystal violet is easily removed due to the thinner layer of peptidoglycan making the cell formal lab report unknown bacteria pink or red.

The domain bacteria is very diverse, making it impossible to formal lab report unknown bacteria it without the use of staining and biochemical tests. Biochemical tests can test for a variety of characteristics such as shape, morphology, pH, etc. Tests such formal lab report unknown bacteria Methyl Red and Voges- Proskauer formal lab report for glucose fermentation. The Citrate Test source a nutrient utilization test that determines whether a bacterium produces the enzyme citrate- permease to be utilized as its sole source of carbon.

Urease test go here used to indicate if a bacterium can hydrolyze the intracellular enzyme urea. SIM gives three test results; Sulfur reduction, which determines whether a bacterium can use enzymes to reduce sulfur to hydrogen sulfide, Indole production formal lab report unknown bacteria to determine if the bacteria can hydrolyze tryptophan to pyruvate, ammonia, and motility when in the semisolid media a motile bacteria can diverge from the stab line.

TSIA is designed to differentiate formal lab report unknown bacteria with reference to glucose fermentation, lactose fermentation, sucrose fermentation, and sulfur reduction Leboffe

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